ngongo CPT 8ml

Whakaahuatanga Poto:

Tauira No.	CPT08
Rauemi	Karaehe Rongoa Neutral
taapiri	Anticoagulant + Gel + Ficoll Solution
Taupānga	Tangohanga Mononuclear Cell mo te rangahau
Rahi ngongo	16*120mm
Tuhia Rōrahi	8ML
Paerewa haumaru	Paerewa Haumaru o te Ao
Hoko Waeine	200 ngongo / kaata
Tae potae	Pango & Kahurangi
Tauira	Kei te waatea
Te Ora Papa	1 Tau
OEM/ODM	Kei te waatea te tapanga, te papanga, te hoahoa kete.
Tiwhikete Kounga	ISO13485, FSC, MDSAP
Whakaaturia	DHL, FedEx, TNT, UPS, EMS, SF, etc.
Utu	L/C, T/T, Western Union, Paypal, etc.

Te takai

Te putea takitahi i roto i te putea pepa;

Tukuna he imeera ki a matou

Taipitopito Hua

Tohu Hua

He Vacutainer CPT (Cell Preparation Tube) 8mL he ngongo kohinga toto motuhake e whakamahia ana mo te wehe i nga pūtau mononuclear (PBMC) mai i te toto katoa. Ka whakamahia nuitia i roto i te whakamaarama mate, rangahau mate hopuhopu, me nga tono haumanu penei i te aro turuki mate me nga whakamatautau i runga i te pūtau.

Nga waahanga matua o te ngongo CPT Vacutainer (8mL):
Kua whakakiia-mua ki te reo whakakeke me te reo rōnaki kiato
Kei roto te Sodium Citrate hei anticoagulant.
Kei roto i te Ficoll™-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-te-a-a-Ficoll ™ te reera whakawehe rōnaki kia ngawari te wehenga o nga pūtau mononuclear.
Ka taea te wehe PBMC kotahi-taahiraa
Ka whakakore i te hiahia mo te raupatu rōnaki kiato a-ringa (hei tauira, Ficoll-Paque™).
Ka whakaiti i te wa tukatuka me te rereketanga.
Kua whakaritea te kaha o te toto toto
I hoahoatia ki te tukatuka ki te 8mL o te toto katoa.
Korekore, ngongo whakamahi kotahi
Ka whakamahia mo nga tono haumanu me te rangahau e hiahiatia ana te wehe PBMC kounga teitei.
Hototahi ki te centrifugation
Ka hiahiatia he kawa centrifugation kia pai ai te wehe i nga PBMC mai i etahi atu waahanga toto.

Whakamahinga me nga tono:
Ko te wehenga PBMC mo nga rangahau mahi aukati (ngā pūtau T, pūtau B, pūtau NK, monocytes).
Rere cytometry, ELISPOT, cytokine assays, and other immunological analysis.
Te rangahau mate hopuhopu me te kano kano.
Te aro turuki i te mate autoimmune.
Nga whakamatautau haumanu me te kitenga koiora.

Me pehea te mahi (Tuhinga Taketake):
1. Kohia he tauira toto ki roto i te ngongo CPT (whakakiia ki te rōrahi kua tohua).
2. Hurihia ata (8–10 wa) ki te uru ki te anticoagulant.
3. Centrifuge rite ia kawa o te kaihanga (te nuinga a tawhio noa 1500-1800 RCF mō te 20-30 meneti i te pāmahana rūma).
4. Ka puta te paparanga PBMC ki runga ake i te whakawehe gel i muri i te centrifugation.
5. Kia ata tango i te paparanga PBMC ma te whakamahi i te pipette mo nga tono whakararo.

Rokiroki me te Mahinga:
Rokiroki ki te pāmahana rūma (15–25°C).
A ape i te wera nui, te mīti ranei.